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. Author manuscript; available in PMC: 2014 Dec 30.
Published in final edited form as: Nat Commun. 2014 Jun 30;5:4209. doi: 10.1038/ncomms5209

Figure 7. Reactivation of the inactive XΔTsix paternal X-chromosome in the inner cell mass (ICM).

Figure 7

RNA FISH detection of Xist (white), Tsix (green), and Atrx (red) RNAs in E4.0 ICMs. Nuclei are stained blue with DAPI. Insets show representative reactivated nuclei. Scale bar, 20 µm. (c) Quantification of the number of reactivated nuclei, as characterized by loss of Xist RNA coating and biallelic Atrx expression, in individual ICMs (n=6 ICMs per genotype). The mean number of reactivated cells per ICM does not differ significantly between XX and XXΔTsix ICMs (p = 0.23, two-tailed T-test). Error bars, S.D. (d) Allele-specific X-linked gene expression analysis in E5.0 epiblast cells. Representative chromatograms of sequenced cDNAs show biallelic expression of the X-linked genes Pdha1, Rnf12, and Utx, regardless of genotype.