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. Author manuscript; available in PMC: 2014 Jul 8.
Published in final edited form as: Exp Eye Res. 2010 Aug 7;91(5):613–622. doi: 10.1016/j.exer.2010.07.019

Table 1. Relative mRNA levels of genes encoding Ca2+ handling proteins in EOM myotubes and EOM tissue compared with TA.

Listed are relative mRNA levels of genes encoding Ca2+ handling proteins determined by SYBR Green qPCR in myotubes and muscle tissue. Mean fold-changes ± SEM of three independent samples each were calculated and statistically analysed using REST 2005 and P < 0.05 was considered statistically significant.


Myotubes Tissue

Protein category Protein Function Gene Fold-change EOM vs TA statistical significance Fold-change EOM vs TA statistical significance

Pumps SR Ca2+ ATPase, fast-twitch Serca1 1.0 ± 0.65 n.s. 1.1 ± 0.20 n.s.
SR Ca2+ ATPase, slow-twitch Serca2 1.2 ± 0.19 n.s. 13.2 ± 6.75 < 0.05
Ca2+ ATPase 1, plasma membrane Pmca1/Atp2b1 1.1 ± 0.20 n.s. 33.3 ± 7.77 < 0.05
Ca2+ ATPase 4, plasma membrane Pmca4/Atp2b4 1.1 ± 0.38 n.s. 30.0 ± 15.40 < 0.05
Na+/Ca2+ exchanger, plasma membrane Ncx/Scl8a1 1.4 ± 0.34 n.s. 15.8 ± 6.68 < 0.05

Regulatory proteins SR, regulates SERCA2 Phospholamban 2.7 ± 0.42 < 0.05 74.7 ± 10.72 < 0.05
SR, regulates SERCA1 Sarcolipin 1.3 ± 0.16 < 0.05 20.6 ± 11.12 < 0.05
cytosol, regulates SERCAs Regucalcin 1.5 ± 0.33 < 0.05 12.7 ± 6.3 < 0.05

SR calcium binding proteins SR, calcium binding protein, skeletal Calsequestrin 1 0.9 ± 0.26 n.s. 1.5 ± 0.39 n.s.
SR, calcium binding protein, cardiac Calsequestrin 2 1.5 ± 0.27 < 0.05 29.9 ± 18.30 < 0.05
SR, calcium binding protein Sarcalumenin 1.4 ± 0.31 n.s. 2.6 ± 0.55 n.s.

Cytosolic calcium binding proteins cytosol, calcium binding protein Parvalbumin 2.0 ± 0.35 < 0.05 2.3 ± 0.54 n.s.
cytosol, calcium binding protein S100a1 2.3 ± 0.39 < 0.05 5.4 ± 0.68 < 0.05
calcium binding protein Calmodulin 0.9 ± 0.10 n.s. 10.1 ± 3.64 < 0.05
calcium binding protein, slow-twitch/cardiac Troponin C 1 1.0 ± 0.14 n.s. 5.1 ± 0.59 < 0.05
calcium binding protein, fast-twitch Troponin C 2 1.4 ± 0.29 n.s. 2.1 ± 0.49 n.s.

Calcium channels calcium channel subunit Cacna1s 1.5 ± 0.22 < 0.05 0.9 ± 0.12 n.s.
calcium release channel, skeletal Ryanodine Receptor 1 1.3 ± 0.25 n.s. 1.0 ± 0.16 n.s.