. Author manuscript; available in PMC: 2014 Jul 8.

Published in final edited form as: Exp Eye Res. 2010 Aug 7;91(5):613–622. doi: 10.1016/j.exer.2010.07.019

Table 1. Relative mRNA levels of genes encoding Ca²⁺ handling proteins in EOM myotubes and EOM tissue compared with TA.

Listed are relative mRNA levels of genes encoding Ca²⁺ handling proteins determined by SYBR Green qPCR in myotubes and muscle tissue. Mean fold-changes ± SEM of three independent samples each were calculated and statistically analysed using REST 2005 and P < 0.05 was considered statistically significant.


			Myotubes		Tissue

Protein category	Protein Function	Gene	Fold-change EOM vs TA	statistical significance	Fold-change EOM vs TA	statistical significance

Pumps	SR Ca²⁺ ATPase, fast-twitch	*Serca1*	1.0 ± 0.65	n.s.	1.1 ± 0.20	n.s.
	SR Ca²⁺ ATPase, slow-twitch	*Serca2*	1.2 ± 0.19	n.s.	13.2 ± 6.75	< 0.05
	Ca²⁺ ATPase 1, plasma membrane	*Pmca1/Atp2b1*	1.1 ± 0.20	n.s.	33.3 ± 7.77	< 0.05
	Ca²⁺ ATPase 4, plasma membrane	*Pmca4/Atp2b4*	1.1 ± 0.38	n.s.	30.0 ± 15.40	< 0.05
	Na+/Ca²⁺ exchanger, plasma membrane	*Ncx/Scl8a1*	1.4 ± 0.34	n.s.	15.8 ± 6.68	< 0.05

Regulatory proteins	SR, regulates SERCA2	*Phospholamban*	2.7 ± 0.42	< 0.05	74.7 ± 10.72	< 0.05
	SR, regulates SERCA1	*Sarcolipin*	1.3 ± 0.16	< 0.05	20.6 ± 11.12	< 0.05
	cytosol, regulates SERCAs	*Regucalcin*	1.5 ± 0.33	< 0.05	12.7 ± 6.3	< 0.05

SR calcium binding proteins	SR, calcium binding protein, skeletal	*Calsequestrin 1*	0.9 ± 0.26	n.s.	1.5 ± 0.39	n.s.
	SR, calcium binding protein, cardiac	*Calsequestrin 2*	1.5 ± 0.27	< 0.05	29.9 ± 18.30	< 0.05
	SR, calcium binding protein	*Sarcalumenin*	1.4 ± 0.31	n.s.	2.6 ± 0.55	n.s.

Cytosolic calcium binding proteins	cytosol, calcium binding protein	*Parvalbumin*	2.0 ± 0.35	< 0.05	2.3 ± 0.54	n.s.
	cytosol, calcium binding protein	*S100a1*	2.3 ± 0.39	< 0.05	5.4 ± 0.68	< 0.05
	calcium binding protein	*Calmodulin*	0.9 ± 0.10	n.s.	10.1 ± 3.64	< 0.05
	calcium binding protein, slow-twitch/cardiac	*Troponin C 1*	1.0 ± 0.14	n.s.	5.1 ± 0.59	< 0.05
	calcium binding protein, fast-twitch	*Troponin C 2*	1.4 ± 0.29	n.s.	2.1 ± 0.49	n.s.

Calcium channels	calcium channel subunit	*Cacna1s*	1.5 ± 0.22	< 0.05	0.9 ± 0.12	n.s.
Calcium channels	calcium release channel, skeletal	*Ryanodine Receptor 1*	1.3 ± 0.25	n.s.	1.0 ± 0.16	n.s.

Table 1. Relative mRNA levels of genes encoding Ca2+ handling proteins in EOM myotubes and EOM tissue compared with TA.

Table 1. Relative mRNA levels of genes encoding Ca²⁺ handling proteins in EOM myotubes and EOM tissue compared with TA.