Staining of collagen fibers was assessed for mice groups: WT C57, WT C3H, MNX
C57, and MNX C3H. (A) Representative photomicrographs of Sirius Red-stained
liver sections are shown WT C57 (a, n=5), WT C3H (c, n=3), MNX
C57 (e, n=4), and MNX C3H (g, n=4) mice fed the control diet;
and WT C57 (b, n=7), WT C3H (d, n=5), MNX C57 (f, n=6),
and MNX C3H (h, n=5) mice fed the atherogenic diet (magnification
× 400). The arrows indicate Sirius Red-stained collagen fibers. (B) Bar
graph representing % area stained with Sirius Red. Results are expressed
as mean ± SE. *p<0.05 or **p<0.0001,
compared to corresponding control diet counterpart. ANOVA results are provided
in Supplemental Table 1
and p values for pair-wise comparisons are provided in Supplemental Table 3.