Table 1.
Effect of diet, genotype, and haplotype on body parameters, liver measurements, and select serum chemistries.
| WT C57
(C57n:C57mt) |
WT C3H
(C3Hn:C3Hmt) |
MNX C57
(C57n:C3Hmt) |
MNX C3H
(C3Hn:C57mt) |
|||||
|---|---|---|---|---|---|---|---|---|
| Control | Atherogenic | Control | Atherogenic | Control | Atherogenic | Control | Atherogenic | |
| Body weight (g) | 28.8 ± 1.3 | 24.9 ± 0.3* | 31.3 ± 0.8 | 42.4 ± 0.4** | 28.5 ± 0.8 | 24.5 ± 1.04* | 32.9 ± 0.8 | 42.4 ± 0.8** |
| Liver weight (g) | 1.4 ± 0.1 | 1.8 ± 0.01* | 1.7 ± 0.02 | 2.9 ± 0.1** | 1.3 ± 0.03 | 2.1 ± 0.1** | 1.5 ± 0.07 | 2.9 ± 0.1** |
| Liver weight/body weight | 4.6 ± 0.1 | 7.3 ± 0.2** | 5.6 ± 0.2 | 6.9 ± 0.2* | 4.7 ± 0.1 | 8.7 ± 0.3** | 4.6 ± 0.2 | 7.6 ± 0.5** |
| Liver TAG (mg/mg prot) | 3.1 ± 0.5 | 5.4 ± 1.4 | 3.9 ± 0.6 | 4.5 ± 0.4 | 2.7 ± 0.07 | 3.5 ± 1.5 | 2.1 ± 0.12 | 3.3 ± 0.05* |
| Serum TAG (mg/dL) | 31.0 ± 2.8 | 20.0 ± 3.8 | 87.4 ± 9.4 | 33.5 ± 4.2** | 21.4 ± 1.2 | 13.2 ± 2.00 | 93.8 ± 11.7 | 63.2 ± 12.4** |
| Total Serum Chol (mg/dL) | 56.5 ± 8.8 | 125.1 ± 9.9* | 148.1 ± 7.7 | 196.9 ± 29.8 | 55.8 ±12.7 | 113.4 ± 12.3* | 169.4 ± 9.1 | 298.4 ± 7.5** |
| Free Serum Chol (mg/dL) | 6.7 ± 1.3 | 16.6 ± 1.5* | 14.9 ± 0.8 | 16.8 ± 0.7 | 7.6 ± 0.9 | 15.5 ± 1.3* | 16.8 ± 1.07 | 18.2 ± 2.4 |
| Serum Adiponectin (μg/mL) | 6.1 ± 0.4 | 5.7 ± 0.7 | 2.8 ± 0.3 | 4.6 ± 0.4* | 8.0 ± 0.5 | 5.7 ± 0.5* | 5.0 ± 0.6 | 3.9 ± 0.4 |
| Serum ALT (IU/L) | 13.9 ± 3.5 | 51.2 ± 4.9* | 22.4 ± 5.2 | 35.5 ± 5.8 | 27.8 ± 4.6 | 88.2 ± 17.9* | 33.9 ± 9.1 | 95.8 ± 11.3** |
Mice were fed a control diet or an atherogenic diet for 12 wk. Data are presented as mean ± SE. The number of mice used for: 1) body weight, liver weight and liver/body weight ratio, n=5–15; 2) liver TAG, n=3–4; 3) serum TAG, n=4–9; 4) total serum cholesterol, n=3–5; 5) free serum cholesterol, n=5–11; 6) serum adiponectin, n=5; and 7) serum ALT, n=4–9. Statistical differences as compared with corresponding control counterparts:
p<0.05;
p< 0.0001 are for differences between dietary treatments (atherogenic diet compared to control diet). Other pair-wise comparisons are included in Supplemental Data Table 3.
Abbreviations: TAG, triacylglycerol; Chol, cholesterol; ALT, alanine aminotransferase.