FIG. 2.

Comparison of hESC-MSCs to bone marrow (BM)-MSCs shows some similarities and some differences. (A) Cumulative population doublings (PD) over time for two hESC-MSC lines (black lines with diamonds and squares) versus two BM-MSC lines from different donors (gray lines with triangles and Xs), starting at passage 0. On harvest, cells were counted, replated at 6,800 cells/cm² for subsequent passages. Cumulative PD were calculated based on PD=log(Nf−Ni)/log 2, by which Nf is number of harvested cells and Ni is number of cells initially plated. (B) Left panel: bar graph showing statistically significant difference in average cell length for BM-MSCs (n=118) and hESC-MSCs (n=105) at passage 5, P<0.0001 using two-tailed Student's t-test. Right panel contains histogram plots showing the slight size difference in FSC (top graph) and SSC (bottom graph) between hESC-MSCs and BM-MSCs at p5. hESC-MSCs are in shaded gray, and BM-MSCs are in shown with unshaded black line. (C) Bar graph showing percentage of MSCs staining positive for indicated cell surface markers using flow cytometry for hESC-MSCs and BM-MSCs. Bars represent average of three to six experiments, and error bars represent standard deviation. Boxes are drawn around markers with significant differences between hESC-MSCs and BM-MSCs (corresponding histogram plots are shown in Supplementary Fig. S1). *Indicates statistically significant P-values (P<0.005 for Stro-1; P<0.002 for CD10, and P<0.02 for CD24). (D) Representative flow cytometry dot plots for hESC-MSCs and BM-MSCs for CD10, Stro-1, and CD24.