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. 2014 Mar 20;23(14):1611–1624. doi: 10.1089/scd.2013.0554

FIG. 3.

FIG. 3.

Dynamic changes in secreted proteins in response to pro-inflammatory stimulation. Quantitative assessment of cytokine concentration in hESC-MSC conditioned medium (CM) in the absence or presence of 50 ng/mL interferon gamma (IFNγ) and/or 25 ng/mL tumor necrosis factor alpha (TNFα) as indicated for 48 h. Human chemokine cytometric bead arrays (BD Bioscience) were used for inducible protein 10 (IP-10), monokine induced by gamma interferon (MIG), interleukin 8 (IL-8), monocyte chemotactic protein 1 (MCP-1), and RANTES; while ELISAs (ebioscience) were used for IL-6, granulocyte macrophage colony-stimulating factor (GM-CSF), and transforming growth factor beta (TGFβ), according to the manufacturer's protocols. Media only was used to determine background in all assays. Bars show the average of three to five independent experiments for each cytokine; error bars are SD; P-values for significance between basal and stimulated (IFNγ, TNFα, or IFNγ+TNFα) states, *P≤0.02; **P≤0.002; ***P≤0.0002.