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Evolution of HES-3 expansion in spinner flasks. Static pauses of 1- and 2 days were introduced for PS MC coated with PLL+LN and PLL+VN, respectively, to stabilize the hESC/MC aggregates before agitation. Parts (A) and (C) depict hESC cell density and the size of hESC/MC aggregates over 9 days' culture, while parts (B) and (D) show the number density of hESC/MC aggregates and the percentage of free MC, for PS MC coated with PLL+VN and PLL+LN, respectively.
