FIG. 9.

Characterization of HES-3 cells expanded in spinner flasks on PS MC with PLL+VN and PLL+LN coatings, respectively. (A) FACS of pluripotent markers. For differentiated HES-3: (B) images of immunochemical staining for markers associated with the three embryonic germ layers, AFP (endoderm), SMA (mesoderm), and β-III tubulin (ectoderm). Scale bars are 200 μm. (C) quantitative RT-PCR data for a pluripotent marker gene and genes associated with the formation of the three primary germ layers. FACS, fluorescence-activated cell sorting; RT-PCR, reverse transcription-polymerase chain reaction.