Table 1.
Kinetics Size of hESC/MC Aggregates Formation, During HES-3 Growth on Coated PS MC (VN, LN, PLL+VN, and PLL+LN) in Three Different Agitation Regimes
| hESC/MC aggregates size (μm) | |||||
|---|---|---|---|---|---|
| Agitation regimes | Days | VN | LN | PLL+VN | PLL+LN |
| 2 days' static | |||||
| 1 | 120±11 | 560±20 | 360±18 | 570±13 | |
| 2 | 200±18 | 710±30 | 600±20 | 620±13 | |
| 3 | 160±13 | 560±14 | 430±20 | 310±10 | |
| 5 | 190±11 | 580±18 | 280±13 | 330±10 | |
| 7 | 230±14 | 610±14 | 290±13 | 340±10 | |
| 1 day static | |||||
| 1 | 130±10 | 610±13 | 370±19 | 600±11 | |
| 2 | 130±10 | 380±10 | 190±10 | 360±10 | |
| 3 | 160±3 | 470±11 | 200±17 | 320±10 | |
| 5 | 190±3 | 510±10 | 210±3 | 330±10 | |
| 7 | 200±11 | 530±11 | 230±10 | 330±10 | |
| 0 day static (continuous agitation) | |||||
| 1 | 120±10 | 470±15 | 140±17 | 280±13 | |
| 2 | 120±13 | 470±18 | 130±10 | 280±10 | |
| 3 | 130±11 | 430±19 | 130±10 | 310±10 | |
| 5 | 150±13 | 370±17 | 160±10 | 320±10 | |
| 7 | 150±30 | 370±14 | 170±10 | 320±11 | |
hESC, human embryonic stem cells; LN, laminin; PLL, poly-L-lysine; PS, polystyrene; VN, vitronectin.