Figure 4.

NaHS suppresses Hcy-upregulated CHOP expression in the hippocampus of rats, and this effect is blocked by k252a. (A) Rats were pretreated with NaHS (30 and 100 μmol·kg⁻¹·d⁻¹ for 2 d, ip) and then co-treated with Hcy (0.6 μmol/d for 7 d, icv). (B) Rats were pretreated with NaHS (30 and 100 μmol·kg⁻¹·d⁻¹ for 2 d, ip) in the absence or presence of treatment with k252a (1 μg/d for 2 d, icv) and then co-treated with Hcy (0.6 μmol/d for 7 d, icv). The expression of CHOP in the hippocampus of rats was detected by Western blotting using an anti-CHOP antibody, and β-actin was used as a loading control. Values are expressed as the mean±SEM (n=3−5). ^cP<0.01 vs control group. ^eP<0.05, ^fP<0.01 vs Hcy-treated alone group. ⁱP<0.01 vs co-treated with Hcy and NaHS group.