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. 2014 May 5;35(6):825–831. doi: 10.1038/aps.2013.195

Figure 3.

Figure 3

With the pre-treatment of DAPT, t-AUCB significantly increased caspase-3 activity. U251 (A) or U87 (B) cells were either untreated, treated only with 200 μmol/L t-AUCB for 72 h, treated only with 2 μmol/L DAPT for 72 h, or pre-treated with 2 μmol/L DAPT for 4 h followed by 72 h-treatment of 200 μmol/L t-AUCB. Caspase-3 activity was measured using an assay kit. Absorbance read at 405 nm represented the level of caspase-3 activity. The results showed that no significant change in caspase-3 activity was observed in cells only treated with 200 μmol/L t-AUCB or 2 μmol/L DAPT for 72 h. In cells with the pre-treatment of DAPT followed by the treatment of t-AUCB, the activity of caspase-3 was significantly greater than in those treated with t-AUCB only. aP>0.05 vs Control. fP<0.01 vs t-AUCB.