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. Author manuscript; available in PMC: 2014 Jul 8.
Published in final edited form as: Methods Mol Biol. 2012;814:327–340. doi: 10.1007/978-1-61779-452-0_21

Fig. 1.

Fig. 1

Reactive astrocytes induced by TGF-β1 produce more CSPGs. (a) Conditioned medium (CM) and cell lysates collected from control or TGF-β1-treated astrocytes at indicated time points were subject to western blotting with the CS-56 antibody. TGF-β 1-treated astrocytes showed increased production of CSPGs detected in CM as well as cell lysates; (b) CM from control or TGF-β1-treated astrocytes was processed for ELISA using antibody CS-56; (c) CM from control or TGF-β1-treated astrocytes was concentrated and digested with cABC followed by western blotting with 2B6 or 3B3 antibody; (d) TGF-β1-treated astrocytes showed increased immunoreactivity with CS-56 antibody as compared to untreated control astrocytes ((a–c) From Wang et al. (4)).