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. Author manuscript; available in PMC: 2014 Jul 8.
Published in final edited form as: Methods Mol Biol. 2012;814:327–340. doi: 10.1007/978-1-61779-452-0_21

Fig. 2.

Fig. 2

Increased produce of CSPGs by reactive astrocytes is responsible for reduced neuron growth. (a) CGNs grown on a monolayer of TGF-β1-treated astrocytes showed a decrease in neurite length compared to that on untreated control astrocytes; (b) CM from control astrocytes, or CM from TGF-β 1-treated astrocytes with or without cABC treatment, or CSPGs (positive control) was immobilized on PLL-coated coverslips for the neurite guidance spot assay. Like CSPGs, TGF-β1-CM formed a strong boundary to neurite crossing, while cABC-treated TGF-β1-CM permitted increased crossing of neurites onto the spot.