FIG. 5.

Mitochondrial autophagy and dynamics in GSNOR-KO models. (A) Representative fluorescence microscopy images of myotubes derived from GSNOR-KO (KO) and wild-type (WT) mice carrying GFP-LC3 in heterozygosis on staining with anti-p62 antibody (red). Colocalization between LC3 and p62-positive spots are indicated (white arrowhead or dotted circle). Scale bar, 10 μm. (B) Western blot analyses of Opa1 and Drp1 performed in tibialis anterior homogenates. LDH was selected as a loading control. (C) Representative fluorescence microscopy images of myotubes derived from KO and WT mice, incubated or not with 5 mM N-acetylcysteine (NAC) for 4 h on staining with JC-1. Scale bar, 100 μm. (D) Representative fluorescence microscopy images of myotubes derived from KO and WT mice incubated or not with 5 mM NAC for 2 h on staining with antibodies anti-p62 (red) and anti-Grp75 to visualize mitochondria (green), and DAPI for nuclei (blue). Scale bar, 10 μm. To see this illustration in color, the reader is referred to the web version of this article at www.liebertpub.com/ars