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. 2012 Dec 3;34(1):167–175. doi: 10.1038/aps.2012.172

Figure 8.

Figure 8

The biological functions of miR-338-3p on CRC-derived cell line. (A) SMO 3′-UTR site potentially targeted by miR-338-3p as predicted by TargetScan. (B) Western-blot analysis showing SMO protein expression in SW-620 cells. β-Actin was used as house-keeping gene to normalize SMO protein expression. Note: lane 1, Negative control; lane 2, SW-620 cells transfected with pLV-THM-control; lanes 3, SW-620 cells transfected with pLV-THM-miR-338-3p-inhibitor. The results are representative of three independent experiments. (C–E) Transwell assay showing migration ability of the blank control SW-620 cells (C), SW-620 cells transfected with pLV-THM-control (D) and SW-620 cells transfected with pLV-THM-miR-338-3p-inhibitor (E). Migrated cells found on the bottom side of the membrane were fixed and stained. Numbers of migrated cells on the membrane bottom were counted and shown at the top of each panel. Down-expression of miR-338-3p increased the expression of SMO protein in SW-620 cells, which showed obviously enhanced invasive ability in transwell assay. n=3. Mean±SD. bP<0.01 versus control group.