FIG. 2.

Morphology and growth rates of iMPCs. (A) Typical cell morphology of M-iMPCs. Scale bar: 200 μm. Insets are fourfold magnifications. A-iMPC morphology was not markedly different from M-iMPCs. (B) Growth rates of BMSCs (black line) in comparison to M-iMPCs (solid gray line) and A-iMPCs (dashed gray line) derived using different procedures (EB, SD, CC, and GM). Values are mean±standard deviation of four to six replicate cell lines for iMPCs and three replicates from the same donor for BMSCs. Results are presented up to passage 6. Analysis was carried out up to passage 18, with all iMPCs ceasing proliferation after passage 9 (data not shown). CC, coculture; EB, embryoid body; GM, growth medium; iMPCs, iPSC-derived mesenchymal stem cell-like progenitor cells; SD, spontaneous differentiation.