FIG. 4.

Mesenchymal gene expression in M-iMPCs compared with BMSCs and M-iPSCs. (A) Heat map of PCR array results for each of the three BMSC, M-iPSC, and M-iMPC-SD, -EB, -CC, and GM replicates. (B) Expression of selected pluriptency-associated genes (top) and MSC-associated genes (bottom) by BMSCs, iPSCs, and iMPCs. Values are the mean of three replicates, and error bars represent 95% confidence intervals. (C) Volcano plots of differentially expressed genes between M-iPSCs and BMSCs (top), M-iMPCs and M-iPSCs (middle), as well as M-iMPCs and BMSCs (bottom). Genes with a>10-fold change (>4-fold for iMPC vs. BMSC) are colored red (over-expressed) and green (under-expressed). The vertical gray lines represent P=0.05, and the vertical blue lines represent significance at the Bonferroni-adjusted P value (P<5.6×10⁻⁴). Fold changes and P values of the individual genes are itemized in Table 2. All 12 iMPC lines that were investigated in the PCR array were grouped together (n=12) for this analysis. (D) Hierarchical cluster analysis (Pearson) of the average linkage between groups. MSC, mesenchymal stem cell. Color images available online at www.liebertpub.com/scd