Table 1.
Primers | Sequence 5′ → 3′ | GC% | Annealing temperature (°C/sec) |
---|---|---|---|
1 | GTC CAT GCCA | 60 | 30/60 |
2 | ACA TCG CCCA | 60 | 30/60 |
3 | ATG CCC CTG T | 60 | 30/60 |
Primers | Sequence 5′ → 3′ | GC% | Annealing temperature (°C/sec) |
---|---|---|---|
1 | GTC CAT GCCA | 60 | 30/60 |
2 | ACA TCG CCCA | 60 | 30/60 |
3 | ATG CCC CTG T | 60 | 30/60 |