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Live cells were stained with green fluorescence by polyanionic dye calcein, and dead cells with red fluorescence were stained with EthD-1 after 4 days in samples of (A) gelatin, (B) heart matrix, and (C) tissue culture plastic (TCP). (D) Quantified NRVM viability. Both heart matrix and gelatin containing hydrogels had viabilities greater than 80% and no significant difference compared to TCP (p>0.05, n=10). Scale bars are 100 μm. Color images available online at www.liebertpub.com/tea
