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. 2014 Mar 24;20(13-14):1971–1979. doi: 10.1089/ten.tea.2013.0491

FIG. 1.

FIG. 1.

Cultured autologous adipose-derived cells and cryoinjured urethral sphincters. (A) At 7 days after culture, the adhered and proliferating adipose-derived cells were spindle shaped. Just before implantation, the cells were labeled with PKH26 (inset). Scale bar: 50 and 20 μm. (B) The cultured cells were positive for STRO1 (yellow). Blue: nuclei. Scale bar: 50 μm. (C, D) The cultured cells (red) were negative for myoglobin (C) and smooth muscle actin (SMA) antibody (D). Blue: nuclei. Scale bar: 50 and 20 μm. (E) At 7 days after injury, the cryoinjured urethra remained opened (arrows). (F) The adipose-derived cells were implanted around the cryoinjured urethra at 3-, 6-, 9-, and 12-o'clock positions (arrows).