FIG. 4.

Differentiation of adipose-derived cells into skeletal muscle-, smooth muscle-, myoblast-, nerve-, and vascular endothelial-like cells. For each triad of images, the PKH26-labeled cells are in the upper left micrograph, the cell-specific marker stain is in the lower left micrograph, and the merged images are in the larger micrograph to the right side of each group. The PKH26-labeled implanted cells were positive for myoglobin (A–C, arrows, scale bar: 10 μm), SMA (D–F, arrows, scale bar: 10 μm), and Pax7 (G–I, arrows, scale bar: 20 μm). Some PKH26-labeled cells were positive for S100 (J–L, arrows, scale bar: 10 μm) and tubulin β3 (M–O, arrows, scale bar: 20 μm). Also, some of the implanted cells were positive for von Willebrand factor (vWF) around the blood vessels (P–R, arrows, scale bar: 20 μm). Blue: nuclei.