Table 2: Composition of different storage media

Medium A was deionized water. It was procured from Milli-Q, Millipore system in the laboratory
Medium B artificial saliva
Ca 1.5 mM		(CaCl2 0.1665 g/l)
PO4 0.9 mM		(NaH2PO4 0.133 g/l)
KCl 150 mM		(KCl 11.184 g/l)
Tris buffer 20 mM		(2.4228 g/l)
NaN3 0.02%
pH was adjusted to 7.0 by addition of dilute HCl and the total solution was made to one liter.
Medium C1 demineralizing solution
Ca 2.0 mM		(CaCl2 0.22 g/l)
PO4 2.0 mM		(NaH2PO4 0.2399 g/l)
Acetate buffer 75 mM		(NaCH3COO 6.152 g/l)
NaN3 0.02%
pH was adjusted to 4.3 by the addition of dilute HCl and dilute NaOH and the total solution was made to one liter.
The composition of remineralizing solution medium C2 was same as artificial saliva.