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. Author manuscript; available in PMC: 2014 Jul 8.
Published in final edited form as: J Struct Biol. 2013 May 13;185(2):215–222. doi: 10.1016/j.jsb.2013.05.003

Fig.2.

Fig.2

A western blot of M18 and variants treated with periodate in the absence and presence of antigen PAD4. α-Flag-HRP was used to detect a Flag-tag on the M18 variants. The shift in mass in the presence of PAD4 corresponds to the mass of PAD4 antigen (∼14 kDa), signaling covalent crosslinking between antibody and antigen. Bands were quantified using ImageJ to determine crosslinking efficiencies, which were listed in Table 1. D17L is the only tested position on M18 light chain. The rest of variants were all on heavy chain.