Fig. 3.
Intranasal immunization with STxB-E7 elicits functional E749–57–specific T cells in cervical and mediastinal lymph nodes. Mice were immunized by the intranasal or the intramuscular route with STxB-E7 or the nonvectorized E7 polypeptide. (A and B) E749–57–specific CD8+ T cells from cervical lymph node (CLN) (A) or mediastinal lymph node (MLN) (B) were detected by tetramer assay or IFN-γ ELISpot directly ex vivo. WT, wild type. For the tetramer analysis, means ± SD obtained with specific tetramer after subtracting values from irrelevant tetramer (always <0.1%) are shown. For the ELISpot analysis, background obtained with cells not pulsed with the E749–57 peptide was also subtracted (always <10 spots per 105 cells). These experiments were reproduced three times with four mice per group. *P < 0.05, **P < 0.01.