FIG. 3.

Effects of TnC and TnR Coating on NBR-induced hMSCs. (A) The mRNA levels of neuronal markers, MAP2 and NFM; (B) glial cell markers, GFAP and MBP; and (C) the synapse marker, synapsin (SYN) were quantified after 7 days of cultivation on surfaces coated with Tns (20 μg/mL) in hMSCs pretreated with NBR for 1 week. Levels were normalized to levels observed in NBR-only treatment (set to 1.0) (presented as a dotted line). NBR-treated hMSCs on TCR-coated dishes promoted neuronal and synaptic gene expression; however, it inhibited GFAP gene expression. Data are presented as the mean±SD of one experiment that was derived from three independent experiments. **p<0.01 (all vs. NBR). nd, not determined.