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. 2014 Jun 23;20(13-14):1908–1921. doi: 10.1089/ten.tea.2013.0188

FIG. 4.

FIG. 4.

Immunofluorescence of TnC and TnR coating on NBR-induced hMSCs. NBR-stimulated hMSCs cultured on Tn-coated surfaces is shown as (A) MAP2 and NFM; (B) glial cell markers, GFAP and MBP; (C) the synaptic marker, synapsin (SYN) and the cytoskeletal marker, β-catenin. The immunoreaction was examined after 7 days of cultivation on Tn-coated surfaces (20 μg/mL) in hMSCs pretreated with NBR for 1 week. DAPI (blue) was used as a counterstain. The DMEM group served as a control. When cells were cultivated on TnC- and TCR-coated surfaces, hMSCs exhibited neurite outgrowth. The white bar represents 50 μm. (D) Percentages of MAP2-positive cells and NFM-positive cells within all DAPI-positive cells; (E) percentages of GFAP-positive cells and MBP-positive cells within all DAPI-positive cells; (F) percentages of SYN-positive cells and neurite-positive cells within all DAPI-positive cells. All data are presented as the mean±SD. *p<0.05, **p<0.01 (all vs. NBR). (G) The cell areas, calculated by the β-catenin-positive cells. DAPI, 4′, 6-diamidino-2-phenylindole.