FIG. 6.

Effects of varied doses and ratios of TnC and TnR mixture (TCR) coating on NBR-induced hMSCs. (A) The mRNA levels of neuronal markers, MAP2 and NFM; (B) glial cell markers, GFAP and MBP; and (C) the synapse marker, synapsin (SYN), were quantified after 7 days of cultivation on TCR-coated surfaces (10–40 μg/mL) in hMSCs pretreated with NBR for 1 week. Levels were normalized to levels observed in NBR-only treatment (set to 1.0) (presented as a dotted line). NBR-treated hMSCs on TCR-coated dishes promoted neuronal and synaptic gene expressions, but inhibited GFAP gene expression in a dose-dependent manner. Data are presented as the mean±SD of one experiment that was derived from three independent experiments. *p<0.05, **p<0.01 (all vs. NBR). (D) The mRNA levels of neuronal markers, MAP2 and NFM; (E) glial cell markers, GFAP and MBP; and (F) the synaptic marker, synapsin (SYN), were quantified after 7 days' cultivation on TCR-coated dishes (different ratios: 3:1, 2:1, 1:1, 1:2, and 1:3, total 20 μg/mL) in NBR-induced hMSCs. Levels were normalized to levels observed in NBR-only treatment (set to 1.0) (presented as a dotted line). Data are presented as the mean±SD of one experiment that was derived from three independent experiments. *p<0.05, **p<0.01 (all vs. NBR). ^#p<0.05, ^##p<0.01 (all vs. TCR 1:1).