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. 2014 Jun 23;20(13-14):1908–1921. doi: 10.1089/ten.tea.2013.0188

FIG. 7.

FIG. 7.

Effects of a TnC and TnR mixture (TCR) coating on NBR-induced hMSCs through the integrins, α7 and α9β1. The mRNA levels of integrins (A) were analyzed after 7 days of cultivation on TCR-coated surfaces (20 μg/mL) in hMSCs pretreated with NBR for 1 week. After adding integrin-blocking antibodies (5 μg/mL), mRNA levels of neuronal markers, (B) MAP2 and NFM; glial cell markers, (C) GFAP and MBP; and the synapse marker, (D) synapsin (SYN), were quantified after 7 days of cultivation on Tn-coated surfaces (20 μg/mL) in hMSCs pretreated with NBR for 1 week. Levels were normalized to levels observed in NBR only treatment (set to 1.0) (presented as a dotted line). Data are presented as the mean±SD of one experiment that was derived from three independent experiments. *p<0.05, **p<0.01 (all vs. NBR). #p<0.05, ##p<0.01 (all vs. NBR+TCR). (E) Inhibition percentages were calculated from (B–D).