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. 2014 Jul 3;11:53. doi: 10.1186/1742-4690-11-53

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Copyright © 2014 Bouwman et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Tat does not associate with miRNAs or with RISC components. A. Clustered heat-map showing the amount of cellular miRNAs in HA-Tat and FLAG-Tat cells and in Ago2, Tat and control IgG IP. Ago2 was immunoprecipitated with two different antibodies (M and S). Samples (columns) and miRNAs (rows) are clustered by their enrichment pattern. miRNA abundance compared to the average across all samples is indicated by colour, according to the scale below (log2 ratio). B. Immunoblotting for Ago1, Ago2, Dicer and HA-Tat in input and HA-Tat IP lysates from HA-Tat cells and control FLAG-Tat cells. The position of size markers are illustrated on the left hand side of each panel.