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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: IEEE/ACM Trans Comput Biol Bioinform. 2013 Sep-Oct;10(5):1234–1240. doi: 10.1109/tcbb.2013.140

Fig. 1.

Fig. 1

Overview of RNA-seq analysis. Reads produced by an RNA-seq experiment are aligned to the genome, then clustered into a graph structure that is traversed to recover all possible isoforms at one locus. Lastly, a subset of transcripts is selected and their abundance quantified from the input reads.