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. Author manuscript; available in PMC: 2015 Jul 24.
Published in final edited form as: Integr Biol (Camb). 2014 May 29;6(7):694–705. doi: 10.1039/c4ib00009a

Fig. 2.

Fig. 2

Sox2+(red)/Hoescht+(blue) nuclei 8 wks after injury. (a) Immunofluorescence of Sox2+ cells from bridge implants delivering lentivirus encoding SHH. Dashed line indicates border between bridge implant and host tissue. Sox2+ immunofluorescence at higher magnification from bridges delivering (b) FLuc, (c) SHH, (d) NT3, or (e) NT3 and SHH. Arrows indicate Sox2+ nuclei. (f) Quantification of Sox2+ nuclei in FLuc, NT3, SHH and NT3+SHH conditions (mean +/− SD). (g) Quantification of sub-populations of Sox2+/Hoescht+ nuclei (co-expression of GFAP or Olig2) as a percentage of total Sox2+/Hoescht+ cells (mean +/− SD). (h) Quantification of sub-population densities of Sox2+/Hoescht+ nuclei (co-expression of GFAP or Olig2) (mean +/− SD).