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. Author manuscript; available in PMC: 2015 Jun 26.
Published in final edited form as: Cell Rep. 2014 Jun 12;7(6):1867–1875. doi: 10.1016/j.celrep.2014.05.024

Figure 3. Commitment to the endocrine lineage is established in ISCs rather than enteroblasts.

Figure 3

(A) Adult specific knock-down of Robo2 in ISCs, using the temperature-sensitive DeltaGal4ts drivers (10 days at 29°C), increases the proportion of prospero-positive cells in the posterior midgut. Similar experiment using the EB-specific Su(H)GBEGal4ts driver does not affect the composition of the intestinal epithelium.

(B,C) Flip-out lineage tracing analysis of the progeny of esgGal4 (ISCs and EBs), DeltaGal4 (ISCs only) and Su(H)GBEGal4 (EBs only) expressing cells, 4 days after induction. GFP+Prospero double positive cells (arrowheads) are found in the progeny of esgGal4 and DeltaGal4 positive cells, but absent from the lineage of Su(H)GBEGal4 expressing cells. n indicates the number of gut analyzed, the numbers between parentheses represent the number of pros+ GFP+ cells / total GFP+ cells.