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. Author manuscript; available in PMC: 2015 Jun 26.
Published in final edited form as: Cell Rep. 2014 Jun 12;7(6):1867–1875. doi: 10.1016/j.celrep.2014.05.024

Figure 4. Prospero expression in ISCs is regulated by the Robo2 pathway.

Figure 4

(A) Representative images of pros+pH3 double positive cells in the posterior midgut of wild-type flies.

(B) Quantification of the proportion of pros+ cells among dividing pH3+ positive cells in control flies or after knocking-down Robo2 expression in esg-positive cells for 10 days. n represents the number of independent experiments, the numbers indicated below the genotypes represents the total number of pH3+ cells counted.

(C) Representative images of esgGal4>mCherry pH3+ pros+ cells.

(D) Representative image of a mitotic cell expressing both the Delta and Prospero proteins (See Figure S3C for additional examples).

(E) Adult specific knock-down of Prospero in ISCs leads to a decrease in the proportion of EEs in the intestine.

(F) Knock-down of Prospero in esg-positive cells suppresses the increased proportion of EEs induced by leaRNAi expression.

n indicates the number of guts analyzed in B, E and F. p-value from two-tailed Student's t-test. NS, Not Significant. See also Figure S3.