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. Author manuscript; available in PMC: 2014 Jul 8.
Published in final edited form as: J Infect Dis. 2010 Jul 1;202(1):39–51. doi: 10.1086/653121

Fig. 4. Mycoplasma-specific cytokine production by cells isolated from the lungs and spleens of infected mice.

Fig. 4

Mononuclear lymphocytes were isolated from the lungs of 14-day mycoplasma-infected wild-type, IFN-γ−/− and IL-4−/− mice. Lung cells were cultured, in vitro, in the absence (grey bars) and presence (black bars) of mycoplasma antigen. Supernatants were collected 4 days later, and the levels of IFN-γ, IL-4 and IL-5 were determined using a Bio-plex suspension array. This experiment was done twice. The lowest detection level for the cytokines was 3.2 pg/ml. Detection was done in samples collected from a total of 7-8 mice/group. Data were evaluated by ANOVA, followed by Fisher protected least square differences multigroup comparison. “*” denotes significant difference (p ≤ 0.05) between groups. “N.D.” denotes signal was not detected.