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. Author manuscript; available in PMC: 2014 Jul 8.
Published in final edited form as: J Infect Dis. 2010 Jul 1;202(1):39–51. doi: 10.1086/653121

Figure 6. Mycoplasma-specific cytokine production by cells isolated from the lungs of immunized mice.

Figure 6

Mononuclear lymphocytes from the lungs were isolated on day 14, following nasal-pulmonary immunizations of wild-type, IFN-γ−/− and IL-4−/− mice. Pooled lung lymphoid cells from 4 mice/group were cultured, in vitro, in the absence (grey bars) and presence (black bars) of mycoplasma antigen. Supernatants were collected 4 days later, and the levels of IFN-γ, IL-4, IL-5 and IL-17 were determined using a suspension array. The lowest detection level for the cytokines was 3.2 pg/ml. Detection was done in samples collected from 2 separate experiments.