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. 2014 Jul 8;9(7):e101471. doi: 10.1371/journal.pone.0101471

Figure 1. Aug6 is localised at the SPB and the spindle.

Figure 1

(A) Sequence alignment of Aug6 proteins from A. nidulans (An), the red bread mould Neurospora crassa (Nc), Homo sapiens (Hs; also called hDgt6/FAM29A/HAUS6), Drosophila melanogaster (Dm; also called Dgt6), and the moss Physcomitrella patens (Pp). Identical amino acids are boxed, and similar ones are hatched. (B) Detection of Aug6-GFP by immunoblotting. A unique band with the expected molecular weight (arrow) was detected by immunoblotting with the anti-GFP antibody in 2 independent GFP-integrated strains (#1 and #2). Asterisks indicate cross-reactions of the antibody with other proteins. The #2 strain was used throughout this study. (C) Time-lapse imaging of Aug6-GFP during mitosis. Images were acquired every 15 s in a single focal plane. Strong signals were detected at the pole of the spindle (yellow), whereas weak punctate signals were observed along the spindle MT (blue). (D) Time-lapse imaging of Aug6-GFP and γ-tubulin-mCherry. They were co-localised at the SPB during mitosis. See also Movie S1. Bars, 2 µm.