Table 1. Plasmids used in this study.
Name | Insert | Marker | Target locus |
pGG857 | aug6Δ construct | argB | aug6 |
pGG858 | Aug6-Cterm-GFP | argB | aug6 |
pGG879 | γ-tubulin-Cterm-mCherry | pyroA | γ-tubulin |
pED106 | Aug2 (AN6003)-Cterm-mCherry | pyroA | aug2 |
pED107 | Aug3 (AN5826)-Cterm-mCherry | pyroA | aug3 |
pED110 | Aug4 (AN10254)-mCherry | pyroA | pyroA |
pED114 | AN0286-mCherry | pyroA | pyroA |
pED120 | Aug3 (AN5826)-Cterm-HAhis | pyroA | aug3 |
pED122 | mCherry-tubulin | pyroA | pyroA |
PCR fragment | Aug1 (AN3724)-Cterm-mCherry | pyroA | aug1 |
aug3Δ construct | pyroA | aug3 | |
AN0286Δ construct | pyroA | AN0286 | |
mad2Δ construct | pyroA | mad2 | |
apsBΔ construct | pyrG | apsB |