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. 2014 Jul 8;9(7):e101471. doi: 10.1371/journal.pone.0101471

Table 1. Plasmids used in this study.

Name Insert Marker Target locus
pGG857 aug6Δ construct argB aug6
pGG858 Aug6-Cterm-GFP argB aug6
pGG879 γ-tubulin-Cterm-mCherry pyroA γ-tubulin
pED106 Aug2 (AN6003)-Cterm-mCherry pyroA aug2
pED107 Aug3 (AN5826)-Cterm-mCherry pyroA aug3
pED110 Aug4 (AN10254)-mCherry pyroA pyroA
pED114 AN0286-mCherry pyroA pyroA
pED120 Aug3 (AN5826)-Cterm-HAhis pyroA aug3
pED122 mCherry-tubulin pyroA pyroA
PCR fragment Aug1 (AN3724)-Cterm-mCherry pyroA aug1
aug3Δ construct pyroA aug3
AN0286Δ construct pyroA AN0286
mad2Δ construct pyroA mad2
apsBΔ construct pyrG apsB