Table 2. PCR primers used in this study.
| Name | Specificity | Sequences |
| aug6 deletion | 5′UTR | TTTGCGGCCGCCCCTAACAAAGGAATTG (NotI) |
| 5′UTR | AAAGGATCCGCTTGCATTTCTATCAGAT (BamHI) | |
| 3′UTR | TTTATCGATGATGGTGATATTGATTGTCTG (ClaI) | |
| 3′UTR | AAAGGGCCCTTCAGGGTCTTTACTCC (ApaI) | |
| GFP-tagging to Aug6's C-terminus | TTTGAATTCGCATTACACCACGCACTCTC (EcoRI) | |
| AAATCTAGACCATCGCCCCCGGGAACGCAA (XbaI) | ||
| mCherry-tagging to γ-tubulin's C-terminus | γ-tubulin* | AAAGGTACCGTTTAAACACGCGTTGATTTATCACCTTG (KpnI/PmeI) |
| γ-tubulin* | AAAGCGGCCGCATACTCCAACTTCATCCTTTC (NotI) | |
| mCherry | AAAGCGGCCGCATGGTGAGCAAGGGCGAGG (NotI) | |
| mCherry | AAAAAGCTTACTTGTACAGCTCGTCC (HindIII) | |
| mCherry-tagging to Aug2's C-terminus | AAAGTTTAAACCCAGGTAACCCGCAATGAGC (PmeI) | |
| TTTGCGGCCGCAATCCGCGACTCCCACATTAC (NotI) | ||
| mCherry-tagging to Aug3's C-terminus | AAAGTTTAAACCAGAAATCTGCCACAGAACC (PmeI) | |
| TTTGCGGCCGCACCCCCACCTTTCCAGGAAC (NotI) | ||
| mCherry-tagging to Aug4's C-terminus | AAAGTTTAAACGATGAAGACGAGGAAGGGAG (PmeI) | |
| TTTGCGGCCGCACCTTTGTAATCGCCGAATCTC (NotI) | ||
| mCherry-tagging to AN0286's C-terminus | AAAGTTTAAACCTCCTCCCTTCCTCTGCATCG (PmeI) | |
| TTTGCGGCCGCATGATTTGAGCGCGGGTCG (NotI) | ||
| HAhis-tagging to Aug3's C-terminus | HAhis | AAAGCGGCCGCATGGTCTTTTACCCATAC (NotI) |
| HAhis | AAAAAGCTTCGAGCTAGTGATGGT (HindIII) | |
| Aug3 | AAAGTTTAAACCAGAAATCTGCCACAGAACC (PmeI) | |
| Aug3 | TTTGCGGCCGCACCCCCACCTTTCCAGGAAC (NotI) | |
| mCherry-tubA | tubA promoter | AAAGGTACCCGTCCGGAATATGCCACTTG (KpnI) |
| tubA promoter | AAAGTTTAAACCTTGTCTAGGTGGGTGGTGA (PmeI) | |
| mCherry | CCCGTTTAAACACCATGGTGAGCAAGGGCGAGGAG (PmeI) | |
| mCherry | TTTGCGGCCGCCTTGTACAGCTCGTCCATGC (NotI) | |
| tubA | TTTGCGGCCGCCACCAGAGAAGTCATTAGTTTG (NotI) | |
| tubA | CACCGGCGCGCCTTAGTACTCAACTTCCTCACC (AscI) | |
| mCherry-tagging to Aug1's C-terminus | Aug1 | AAAGTTTAAACCAGACGAAGAGGCAGCGATG (PmeI) |
| Aug1 | TTTGCGGCCGCAACGTCTGTCCGACAAGTTC (NotI) | |
| 3′UTR | GCCAAAAACCCGTATACTCCTGGA GGATGATCTACTCTTTCCCTAC (pyroA) | |
| 3′UTR | GAACCGTCACATTGAGCTCG | |
| aug3 deletion | 5′UTR | CTAGCCGCTTCGCTAATGCA |
| 5′UTR | GCTTTCGCGCACTTCTGCAG GTCTTGGCGCCAGCGTTGAA (pyroA) | |
| 3′UTR | GCCAAAAACCCGTATACTCCTGGA TGTAAGAATTGGTGCCAAACTGG (pyroA) | |
| 3′UTR | CCCACCTGGCTTTTGCAAGA | |
| AN0286 deletion | 5′UTR | CATTGGGTGAGCCGAGTTCT |
| 5′UTR | GCTTTCGCGCACTTCTGCAG GTTGAGATCACAGCTTTAGCTGC (pyroA) | |
| 3′UTR | GCCAAAAACCCGTATACTCCTGGA GAGAGACAGTCCAACCAACCAAT (pyroA) | |
| 3′UTR | CGATCCCCAATAGTCCAGCA | |
| mad2 deletion | 5′UTR | GACATACGCGGGAATCTTCT |
| 5′UTR | GCTTTCGCGCACTTCTGCAG TTTGTTCGTATATGCTGGATGAGA (pyroA) | |
| 3′UTR | AACCCGTATACTCCTGGATCC ATGTGGCATGGTATCAAGACA (pyroA) | |
| 3′UTR | AGATGAACAGGCAATCTACGG | |
| apsB deletion | 5′UTR | AGGGTGCACATCAGTGACTG |
| 5′UTR | CTTTCGACAGGTATCGAATTC GGTTACGGAGCAGAAAGCGTC (pyrG) | |
| 3′UTR | AATCTGGTAGACAAGCACTGA TCCTCTTTCTTGCTACATATTCG (pyrG) | |
| 3′UTR | CCAGCAAGCGCTTGAAACAT |
The underlines indicate the restriction enzyme sites introduced for ligation.
*After cloning the γ-tubulin-mCherry fusion gene into the pyroA vector, the N-terminal region of γ-tubulin was deleted by PmeI/SmaI digestion. The resultant plasmid was linearised by HpaI and transformed.