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. 2014 Jul 8;9(7):e101333. doi: 10.1371/journal.pone.0101333

Figure 9. Exploring the dominant factor of the intrinsic mitochondria-dependent apoptosis induction.

Figure 9

(A) Relationship between the final concentration of caspase3 and the p53pp-dependent synthesized Bax mRNA abundance. (B) Relationship among the number of p53 pulses, the final concentration of caspase3 and the p53pp-dependent synthesized Bax mRNA abundance. Closed symbol of red, green and blue: IR dose of 0.3, 2.5 and 6.0 Gy, respectively; solid line: regression curve by Hill equation (Hill coefficient = 7.91); broken line: half maximal effective chemical species (Bax) abundance (EC50) to final concentration of caspase3. EC50 was equal to 45816. Caspase3 activation required the generation of multiple p53 pulses. There was individual variability in the number of p53 pulses in cells which were subjected to the same IR dose. The individual variability affected the intrinsic mitochondria-dependent apoptosis induction.