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. Author manuscript; available in PMC: 2014 Oct 3.
Published in final edited form as: Nature. 2014 Jan 29;508(7494):66–71. doi: 10.1038/nature13007

Figure 1. Global measurement of poly(A)-tail lengths.

Figure 1

a, Outline of PAL-seq. For each cluster, the fluorescence intensity reflects the tail length of the cDNA that seeded the cluster. Although the probability of incorporating a biotin-conjugated dU opposite each tail nucleotide is uniform, stochastic incorporation results in a variable number of biotins for each molecule within a cluster. b, Median streptavidin fluorescence intensities for two sets of mRNA-like molecules with indicated poly(A)-tail lengths, which were added to 3T3 (circle), HEK293T (triangle), and HeLa (square) samples for tail-length calibration.