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. 2014 Jul 8;9(7):e101748. doi: 10.1371/journal.pone.0101748

Figure 7. Nuclear β-catenin is absent in the CM and RPE during FGF2-induced regeneration.

Figure 7

Co-expression of nuclear β-catenin (Nu β-cat) and Sox2 in the CM (A–F) and RPE (G–L) at 1 d PR (A–C and G–I) and 3 d PR (D–F and J–L); (B,C) show the boxed area in (A); (E, F) show the boxed area in (D); (H, I) show the boxed area in (G); and (K, L) show the boxed area in (J). Panels A, D, G, and J have DIC overlay. Panels B, E, H, and K include DIC overlay and are equivalent to C, F, I, and L respectively. b = FGF2 bead; Cr = ciliary regeneration; Td = transdifferentiation; PE: pigmented epithelium; NPE: non-pigmented epithelium; RPE: retina pigmented epithelium; L: lens. DAPI stains the nuclei in C, F, I and L. Scale bar in (A) represents 100 µm and applies to D, G and J. Scale bar in (B) represents 100 µm and applies to C, E, F, H, I, K and L.