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. 2014 Jul 8;9(7):e101748. doi: 10.1371/journal.pone.0101748

Figure 11. Canonical Wnt signaling inhibitor XAV939 induces chick retina regeneration.

Figure 11

(A–F) Patterns of nuclear β-catenin (Nu β-cat) 24 hours after intravitreous injection of Wnt signaling inhibitor XAV939 into an E3 developing chick eye (B) compared to the contralateral non-injected eye (A). Close up images of the CM (C, D) and the RPE (E, F) of the non-injected eye (C, E) and the treated eye (D, F). (G) Quantitative analysis shows the difference in regeneration observed in histological sections of XAV939 treated eyes vs PBS treated eyes at 3 d PR (p values shown represent significance). Error bars represent S.E.M. (H–L): Histological sections of eyes treated with PBS (H) or XAV939 (I–L) 3 d PR showing regeneration from the CM (I) and transdifferentiation (J–L). (K) Shows the boxed area in (J). Cr = ciliary regeneration; Td = transdifferentiation; L = lens. Scale bar in (A) represents 100 µm and applies to (B). Scale bar in (H) represents 200 µm and applies to (I) and (J). Scale bar in (K) represents 50 µm. Scale bar in (L) represents 100 µm.