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. 2014 Jul 8;9(7):e101829. doi: 10.1371/journal.pone.0101829

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© 2014 Dorfman et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Three days after vehicle or STZ injection, adult Wistar rats were housed in a standard laboratory cage (panel A) or EE cages (panel B). Panel C: Experimental groups and protocols for functional, histological and biochemical studies. ERG was weekly recorded until week 10. Vascular permeability, GFAP immunostaining, retinal TNFα and TBARS levels were analyzed in SE- or EE-housed animals that had been diabetic for 6 weeks. VEGF levels were analyzed by Western blot and immunohistochemistry at 6 or 10 weeks of diabetes. Panel D: Diabetic animals housed in SE until week 7 were switched to EE, and ERG was weekly recorded until week 10.