Table 1. Coverage and sequencing efficiency of the twelve samples sequenced by the HT-TREBS technique.
Sequenced sample | Number of mapped sequence reads that contain IAP LTR sequences and unique sequences * | Number of IAP LTR loci retained after filtering | Total number of CpG positions sequenced at depth of >15x | Average number of CpG positions sequenced per IAP LTR loci | Average coverage of the CpG positions |
Female #1 Brain | 992,155 | 6,525 | 49,856 | 7 | 123x |
Female #1 Liver | 787,780 | 6,403 | 48,543 | 7 | 100x |
Female #1 Kidney | 904,991 | 6,996 | 53,311 | 7 | 107x |
Female #2 Brain | 2,227,792 | 7,070 | 54,297 | 7 | 250x |
Female #2 Liver | 719,417 | 6,446 | 49,590 | 7 | 96x |
Female #2 Kidney | 926,852 | 7,055 | 54,005 | 7 | 109x |
Male #1 Brain | 712,576 | 6,875 | 52,614 | 7 | 89x |
Male #1 Liver | 963,293 | 6,680 | 51,221 | 7 | 125x |
Male #1 Kidney | 1,111,838 | 7,218 | 55,669 | 7 | 129x |
Male #2 Brain | 1,643,920 | 6,957 | 52,830 | 7 | 178x |
Male #2 Liver | 1,118,820 | 7,109 | 53,775 | 7 | 121x |
Male #2 Kidney | 659,375 | 7,914 | 43,497 | 6 | 71x |
*Number of reads with IAP LTR sequences that mapped to the reference genome and had at least 10 bases of unique sequences.