Table 2. Restriction site associated DNA (RAD) sequence dataset used for evaluation of the pipeline.
Genotype name | Total number of reads (SE) | Read length (bp) | Total number of filtered reads | Read length (bp) | Alignment (%) |
ICCV 03107 | 2,360,400 | 100 | 2,250,687 | 78 | 91.27 |
ICC 4918 | 5,761,446 | 100 | 5,486,801 | 78 | 89.82 |
ICC 4930 | 10,595,164 | 100 | 10,103,218 | 78 | 89.90 |
ICC 4958 | 10,874,599 | 100 | 10,400,166 | 79 | 91.83 |
ICC 5270 | 8,198,607 | 100 | 7,790,524 | 78 | 90.38 |
ICCV 05530 | 8,011,084 | 100 | 7,611,453 | 78 | 89.96 |
ICC 5810 | 8,587,698 | 100 | 8,213,783 | 79 | 90.68 |
ICC 5912 | 5,422,669 | 100 | 5,183,888 | 79 | 91.12 |
ICC 6263 | 8,167,648 | 100 | 7,808,763 | 79 | 90.89 |
ICC 8261 | 6,245,558 | 100 | 5,943,309 | 81 | 88.94 |
Raw reads from above mentioned datasets were filtered and then aligned against the chickpea reference genome. The reads cover 88% to 92% of the reference genome. The pre-processing step of pipeline trimmed 100 bp reads into single end reads in the range 78 bp to 81 bp.