Figure 5.

(A) Coomassie blue stained cultures illustrating the influence of gravity on the distribution of fibroblasts within horizontally (0° or 180°) placed mini-chambers, 1 h after seeding. (B) Coomassie blue stained cultures illustrating the influence of gravity on the distribution of fibroblasts within tilted (45°, 90°) mini-chambers, 1 h after seeding. (C) The influence of fluid flow, microstructure, chamber orientation, and gravity on the distribution of cells within mini-chambers tilted at 45° or 90° over time. The black arrows indicate the direction of gravity (g), and the white arrow indicates the direction of fluid flow (f). The micrographs to the right in the same row were taken at different time points (0, 10, 20, and 40 min). The schematic diagram of the chamber cross-section to the left in each row illustrates the mini-chamber orientation, and the gray frame indicates at which layer the micrograph to the right in the same row was taken after the cells were introduced. Scale bar: 100 μm.