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. Author manuscript; available in PMC: 2014 Jul 8.
Published in final edited form as: Cancer Res. 2009 Apr 7;69(8):3501–3509. doi: 10.1158/0008-5472.CAN-08-3045

Figure 4. Promotion and inhibition of gastric tumor growth and metastasis by FoxM1b overexpression and knockdown.

Figure 4

(A) Untreated N87 cells (Ctrl) and N87 cells transfected with pcDNA3-FoxM1b (M1b) or control pcDNA3 (Neo) were injected into the subcutis (A1) or stomach wall (A2) of nude mice (n = 5). (B) Untreated N87 cells (Ctrl) and N87 cells treated with FoxM1b-siRNA (si-M1b) or control siRNA (si-Ctrl) were injected into the subcutis (B1) or stomach wall (B2) of nude mice (n = 5). The resulting subcutaneous gastric tumors were measured, the mean tumor volume (± SD) in each group of mice was calculated, and representative tumor photos were taken (A1 and B1). The tumors were weighed 60 days after the tumor-cell injection or when animals became moribund, and the mean tumor weight (± SD) in each group of mice was calculated (A2 and B2). This was one representative experiment of three with similar results. (C) Representative liver sections with and without gastric cancer metastases (marked by arrowhead) obtained from a mouse with control N87 cells growing in the stomach wall C1) and from a mouse with FoxM1b-overexpressing N87 cells growing in the stomach wall (C2).