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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Nat Med. 2014 Jun 22;20(7):754–758. doi: 10.1038/nm.3589

Figure 1. Chronic stress increases proliferation of hematopoietic stem and progenitor cells in the bone marrow.

Figure 1

a, Cohen’s perceived stress scale score in medical ICU residents (n = 10–15, Student’s t–test). b, Blood leukocytes in residents (n = 29, Wilcoxon test). c, Leukocytes in mouse blood and d, bone marrow after 3 weeks of stress (n = 9 per group, Student’s t–test). e, Gating for LSK and HSC. Proliferation was assessed after 3 weeks of stress (n = 5 per group, Mann–Whitney test). f, Bone marrow colony forming unit (CFU) assay after one week of stress (n = 5 per group, Mann–Whitney test). g, Bone marrow HSC and LSK after 3 weeks of stress (n = 10 per group, Student’s t–test). h, Long–term competitive repopulation assay using limiting dilutions of whole bone marrow from stressed or non–stressed mice (Poisson statistics for LT–HSC frequencies, P = 0.2 two–tailed t–test). i, Competitive reconstitution with 2×106 bone marrow cells from stressed or non–stressed controls co–injected with equal numbers of naive competitor cells, followed by assessment of blood chimerism (n = 10 mice per group, one–way ANOVA). Mean ± s.e.m., * P < 0.05.