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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Nat Med. 2014 Jun 22;20(7):754–758. doi: 10.1038/nm.3589

Figure 2. Hematopoietic progenitors in the bone marrow of stressed mice dilute DiD membrane dye faster.

Figure 2

a, Intravital microscopy of the mouse calvarium after adoptive transfer of DiD–labelled LSK (white arrows) before and seven days after stress (n = 5 mice per group, Mann–Whitney test). Dotted lines outline bone. Scale bar indicates 50 μm. Single dots in graphs represent DiD+ cells’ target–to–background ratio before (upper panel) and after stress (lower panel). b, DiD fluorescence on day 0 and 7 days after adoptive transfer of DiD+ GFP+ LSK in non–stressed control (CT) or stressed mice (n = 5 per group). The bar graph illustrates the DiD fluorescence in GFP+ LSK (Mann–Whitney test). Mean ± s.e.m., * P < 0.05.